(Monday’s/9.30H/CEB anfitheatre)
Daniela Correia, Helicobacter pylori growth under defined conditions
Helicobacter pylori is known to be associated with gastric diseases. The lack of physiological data has hampered the uncovering of mechanisms associated with H. pylori infection and consequently, many aspects related with the appearance of diseases remain unclear. The development of robust methods to grow this bacterium and the identification of the nutritional requirements is of major importance for a better characterization of its physiology. As such, the purposes of this work were the optimization of the culture conditions and the analysis of extracellular metabolites in order to collect data that can help to better understand the metabolism of H. pylori. Response Surface Methodology (RSM) was used as a statistical technique to reach the optimal growth conditions. For the analysis of extracellular metabolites a GC/MS method was applied.
