(Monday’s/9.30H/CEB anfitheatre)
Ana Costa, Evaluation of the OSCARTM system for the production of monoclonal antibodies by CHO-K1 cells
Mammalian cells are used for the production of recombinant proteins, by using gene expression systems to insert the protein gene into the cells and promote production. The most traditional systems are costly and time-consuming and, consequently, new systems are emerging, such as OSCARTM. OSCARTM is said to be faster and provide higher and more stable production yields. However, deeper evaluation of its applicability in a wider range of cells is necessary. So, this study evaluated OSCARTM system for monoclonal antibody expression by a CHO cell line. OSCARTM proved to be reasonably fast and simple, without negative impact on cell growth characteristics. Selection of the minigene was a critical step, with only one minigene working with the cell line assessed. High levels of production were initially obtained but decreased abruptly in the first weeks, remaining relatively stable after. Additionally, this study demonstrated that initial clone selection should rely on productivity analysis.
Oscar Dias, Genome-scale metabolic models
Over the years several genome-scale metabolic models were released with compartment information. Models such as, iMH805/775 (15 compartments), iMM904 (8 compartments) or iRS1563 (6 compartments) include reactions performed in specific cellular organelles, such as mitochondria, chloroplasts (in photosynthetic organisms), lysosomes, cell nucleus or the Golgi apparatus, etc. However, metabolic compounds have to cross, cellular or organelles, membranes so that reactions can take place. Thus, cells have specific structures, the transport systems, to assist on the metabolites relocation.
Hence, work we propose a system to detect and classify potential transport proteins for a given genome.
